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Table 1 Flow cytometry analysis of live Pfnek-4-GFP transfectants grown in standard or culture conditions inducing commitment to sexual differentiation

From: The Plasmodium falciparum, Nima-related kinase Pfnek-4: a marker for asexual parasites committed to sexual differentiation

 

Culture conditions

Multinucleated parasites*

GFP positive (%)

GFP negative (%)

Exp. 1

Standard

5.8 ± 0.4

59.4 ± 0.5

 

Day-5 Gcyte induction

11.3 ± 0.8**

57.0 ± 0.5

Exp. 2

Standard

4.6 ± 0.3

48.3 ± 1.9

 

Day-5 Gcyte induction

9.3 ± 0.8**

46.3 ± 2.9

  1. *Set as the parasite population with Hoechst fluorescence intensity > 2–3 fold higher than that of the ring-stage (1n) parasite population; values are mean ± SD calculated from three separate cultures obtained in two independent experiments at day-5 of culture.
  2. **Paired t-test: two-tailed p value < 0.0001.
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Malaria Journal

ISSN: 1475-2875

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