COPAS-assisted analysis of larval populations. The progeny of DSX/+ mosquitoes, containing non-transgenic as well as heterozygous and homozygous transgenic mosquitoes, was analysed by COPAS. The left diagram (Extinction vs Time of Flight) shows all detected objects. Mosquito larvae were empirically determined to be located inside the gated area (R1). The right diagram (red vs green fluorescence) decomposes the larval population into five categories: non-transgenic (+/+, R6), heterozygous females (XX; DSX/+, R5), heterozygous males (XY; DSX/+, R4), homozygous females (XX; DSX, R3), homozygous males (XY; DSX, R2), as indicated.