Figure 4

A, B, C, D Image analysis of sorted red blood cells infected with GFP-expressing transgenic parasites from line 164/GFP. A. Microscopic image of sorted red blood cells infected with GFP-expressing transgenic parasites from line 164/GFP (cytospin, Giemsa staining, Objective ×100/1.3 Oil). B. Gallery of images of sorted red blood cells infected with GFP-expressing parasites (line 164/GFP) acquired with Imagestream 100 imaging cytometer. Left channel: bright field, middle channel: fluorescent channel 3 (GFP-channel), right channel: merged bright field and green channels. C. Histogram of unsorted red blood cells infected with GFP-expressing transgenic parasites from line 164/GFP. D. Histogram of sorted red blood cells infected with GFP-expressing transgenic parasites (164/GFP).