Heterologous immunization regimens affect the epitope fine specificity of humoral responses in BALB/c mice. Fine specificities of the post boost sera were tested for reactivity against 3D7 (Panel A) and FVO (Panel B) protein fragments and analysed by particle-based flow cytometry (LuminexTM). Proteins coupled to the Luminex beads are shown on the X-axis. Y-axis = mean fluorescence intensity (MFI). Data are from two independent immunization experiments (n = 20/group).