Localization of P. falciparum SHMT isoforms. Parasites were transfected with pGFP (A), pRL_Pf cSHMT (B), pGL_Pf mSHMT (C), and pGL_N24del Pf mSHMT (D) plasmids expressing fluorescent signals from GFP or DsRed. Schematic diagrams of the recombinant plasmids used are shown alongside the confocal micrographs. Mitochondrion and nucleus is stained with Mitotracker™ (red) and Hoechst 33258 (blue) dye respectively. DIC, differential interference contrast image; rep20, rep20 sequence; bsd, blasticidin S deaminase; hDHFR, human dihydrofolate reductase; gfp, green fluorescence protein; DsRed, red fluorescent protein.