Figure 1

Timeline of mosquito transmission of Plasmodium chabaudi chabaudi . Prior to infection, Anopheles stephensi have continuous access to F/PABA from day −5 to day −1 and, following infection, from day 1 to day 14; F/PABA is replaced at ≤48-hour intervals. Images are shown for reference: P. c. chabaudi AS ring (day −14) and macro- (top) and micro- (bottom) gametocyte (day 0) infected erythrocytes isolated from wild-type C57BL/6 mice, stained with Giemsa’s working solution and visualized with light microscopy; P. c. chabaudi AS oocyst (day 8) and sporozoite (day 15) isolated from An. stephensi and visualized with phase contrast microscopy.