Purified P. falciparum and P. vivax sporozoites remain infectious in vitro . A. HC-04 cells were infected with unpurified or purified P. falciparum sporozoites for 24 hours and were then subjected to an IFA (green: anti-PfCSP (2A10), blue: DAPI). Scale bar is 10 microns. B. Representative plots of sporozoite infectivity are provided from flow cytometric measurements of anti-Pf CSP antibody staining of HC-04 cells C. A graphical representation of two biological replicates of infections from (B) demonstrates that there is no infectivity defect of purified sporozoites. D. Purified P. vivax sporozoites were observed by IFA (green: anti-PvCSP 210 or anti-PvCSP247, blue: DAPI). No gross differences in parasite morphology were detected. Scale bar is 5 microns. E. HC-04 cells were infected with unpurified and purified P. vivax sporozoites for four days and developing liver stage parasites were observed by IFA (green: anti-PbHsp70, red: Phalloidin, blue: DAPI). No gross differences in parasite morphology were detected. Scale bar is 5 microns.