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Table 2 Identification of recombinant salivary proteins by mass spectrometry

From: Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites

Band numbera

Accession number (NCBi)

Protein Name

Theoretical MW (kDa)

Number of MS/MS peptide sequences

Sequence coverage (%)

Mascot score

I

gi|13537666

gSG6 protein [An. gambiae]

13.10

9

56.5

1929

II

gi|114864550

gSG6 salivary peptide [An. funestus]

13.06

4

25.4

205

III

gi|4582528

putative 5′-nucleotidase [An. gambiae]

63.46

29

48.9

3703

IV

gi|114864746

5′ nucleotidase [An. funestus]

16.10

6

49.6

819

  1. a The band number corresponds to the same Roman numeral numbers as indicated in Figure2.
  2. The identities of the bands, their NCBi accession numbers, the theoretical molecular weight values, as well as the number of peptide sequences identified, the corresponding percentage sequence coverage and the Mascot score are listed for MS/MS analysis (Individual ions scores > 16 indicate identity or extensive homology and were considered to be significant (p<0.05).