Exploiting the behaviour of wild malaria vectors to achieve high infection with fungal biocontrol agents

Malaria Journal

Table 2 Number of female Anopheles gambiae s.l. collected nightly from each treatment of the five experimental hut trials, the proportion that showed fungal growth after death (Sporulation %) and the average time to death (g) derived from the Weibull models in days (see Additional file 1)

Trial	Design	Treatment	Dosage (conidia/m²)	Average catch per night	Sporulation (%)	Avg. time to death (g)
Trial	Design	Treatment	Dosage (conidia/m²)	Average catch per night	Sporulation (%)	All	Uninfected	Infected
1	Eave netting	Bb I93-825	2*10¹⁰	1.1 ± 0.35	0.0	nd
		Oil control		1.0 ± 0.24	0.0	nd
		Open eaves (control)		45.0 ± 7.8	0.0	nd
2	Eave curtain	Ma ICIPE-30	2*10¹⁰	36.4 ± 6.0	0.0	15.6
		Bb I93-825	2*10¹⁰	32.4 ± 4.4	0.0	17.2
		Oil control		38.4 ± 4.1	0.0	18.4
3	Eave curtain	Bb I93-825 on curtain	8*10¹⁰	27.9 ± 1.7	18.3	18.5
	& panels	Bb I93-825 on panels	8*10¹⁰	30.2 ± 1.3	10.7	17.3
		Open eaves (control)		33.9 ± 1.2	0.0	20.6
4	Eave baffles	Ma IP 46	4.1*10¹⁰	40.6 ± 8	69.1		20.7	11.5
		Bb I93-825	4.1*10¹⁰	56.3 ± 11.4	67.9		17.9	10.5
		Oil control		74.2 ± 14.5	2.0		20.3
5	Bed net strips	Ma IP 46 - long	5*10¹⁰	24.3 ± 3.2	75.5		24.5	10.5
		Ma IP 46 - short	5*10¹⁰	38.4 ± 6.8	74.6		23.0	11.8
		Open eaves (control)		86.2 ± 12.7	3.3		19.2

As sporulation rates in the trials 2 and 3 were zero or very low, we only calculated separate 'average time to death' for fungus-infected individuals (those that sporulated) and fungus-uninfected individuals (those that did not sporulate) of trials 4 and 5. In trial 1, the number of mosquitoes collected in exit traps of treatment huts was too low to calculate 'average time to death' ('nd' in Table). Ma: Metarhizium anisopliae; Bb: Beauveria bassiana

ISSN: 1475-2875