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Volume 11 Supplement 1

Challenges in malaria research

  • Poster presentation
  • Open Access

Anti-plasmodial action of de-novo-designed, cationic, lysine-branched, amphipathic, helical peptides

  • 1,
  • 1 and
  • 1
Malaria Journal201211 (Suppl 1) :P55

https://doi.org/10.1186/1475-2875-11-S1-P55

  • Published:

Keywords

  • Malaria
  • Plasmodium
  • Plasmodium Falciparum
  • Resistance Index
  • Dimeric Version

Background

A lack of vaccine and rampant drug resistance demands new anti-malarials under such circumstances antibiotic peptides may offer a novel approach to tackle the parasite.

Methods

In vitro blood stage anti-plasmodial properties of several de novo-designed, chemically synthesized, cationic, amphipathic, helical, antibiotic peptides were examined against Plasmodium falciparum using SYBR Green assay. Mechanistic details of anti-plasmodial action were examined by optical/fluorescence microscopy and FACS analysis.

Results

Unlike the monomeric decapeptides {(Ac-GXRKXHKXWA-NH2) (X= F,ΔF) (Fm ΔFm IC50 >100 µM)}, the lysine-branched,dimeric versions showed far greater potency {IC50 (µM) Fd 1.5 , ΔFd 1.39}. The more helical and proteolytically stable ΔFd was studied for mechanistic details. ΔFq, a K-K2 dendrimerof ΔFm and (ΔFm)2 a linear dimer of ΔFm showed IC50 (µM) of 0.25 and 2.4 respectively. The healthy/infected red cell selectivity indices were >35 (ΔFd), >20 (ΔFm)2 and 10 (ΔFq). FITC-ΔFd showed rapid and selective accumulation in parasitized red cells. Overlaying DAPI and FITC florescence suggested that ΔFd binds DNA. Trophozoites and schizonts incubated with ΔFd (2.5 µM) egressed anomalously and Band-3 immunostaining revealed them not to be associated with RBC membrane. Prematurely egressed merozoites from peptide treated cultures were found to be invasion incompetent.

Conclusion

Good selectivity (>35), good resistance index (1.1) and low cytotoxicity indicate the promise of ΔFd against malaria.

Authors’ Affiliations

(1)
Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

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