Genetic diversity and signatures of selection of drug resistance in Plasmodium populations from both human and mosquito hosts in continental Equatorial Guinea

Malaria Journal

Table 2 Statistics of the 15 STR loci of Plasmodium falciparum -positive individuals: mutants to PYR

Microsatellites		Sampled populations - Miyobo										Sampled populations - Ngonamanga
		Single mutant		Double mutant		Triple mutant		All samples				Triple mutant
		( N=10)		(N=37)		(N=17)		(N=64)				(N=82)
		R _s	H _e	R _s	H _e	R _s	H _e	R _s	H _e	F _ST	P	R _s	H _e
*Loci* flanking *dhfr* gene	Dhfr 0.3	3	0.51	3	0.32	2	0.15	3	0.33	0.02	NS	7	0.26
	Dhfr 4.4	1	0.00	2	0.14	2	0.17	2	0.10	-0.04	NS	3	0.10
	Dhfr 5.3	3	0.56	2	0.18	1	0.00	2	0.24	0.10	0.03	3	0.08
	All loci	2	0.36	2	0.21	2	0.11	2	0.22	0.03	0.05	4	0.15
Neutral *loci*	TA1	4	0.82	5	0.83	6	0.89	6	0.85	0.04	0.04	12	0.87
	TA102	4	0.64	7	0.88	5	0.84	6	0.79	0.03	NS	8	0.78
	ARA2	4	0.69	5	0.77	6	0.79	5	0.75	-0.03	NS	9	0.81
	TA87	4	0.87	6	0.85	5	0.81	6	0.84	0.01	NS	9	0.84
	PfPK2	5	0.93	6	0.86	5	0.85	6	0.88	-0.03	NS	12	0.87
	TA81	4	0.87	5	0.81	4	0.71	5	0.80	-0.04	NS	10	0.87
	TA42	3	0.60	2	0.11	2	0.28	2	0.32	0.06	NS	8	0.36
	Pfg377	3	0.67	3	0.56	3	0.65	3	0.62	-0.04	NS	7	0.55
	TA109	5	0.86	5	0.80	4	0.79	5	0.82	0.04	0.03	8	0.75
	All loci	4	0.77	5	0.72	4	0.73	5	0.74	<0.01	NS	9	0.74

N: number of isolates genotyped, He: expected heterozygosity; Rs: allelic richness. All loci: mean over loci Rs and He and global Fst over loci as calculated by FSTAT. P: P- values of permutation tests to assess significance of Fst values. NS: non-significant (P>0.05).