Genetic diversity and signatures of selection of drug resistance in Plasmodium populations from both human and mosquito hosts in continental Equatorial Guinea

Table 3 Statistics of the 15 STR loci of Plasmodium falciparum -positive individuals: mutants to SFX

Microsatellites		Sampled populations - Miyobo								Sampled populations - Ngonamanga
		Single mutant		Double mutant		All samples ( N=60)				Double mutant		Triple mutant		All samples (N=85)
										(N=63)		(N=22)
		(N=17)		( N=43)
		R _s	H _e	R _s	H _e	R _s	H _e	F _ST	P	R _s	H _e	R _s	H _e	R _s	H _e	F _ST	P
*Loci* flanking *dhps* gene	Dhps 0.8	4	0.60	5	0.75	5	0.67	0.02	NS	9	0.81	5	0.64	9	0.73	0.06	0.01
	Dhps 4.3	6	0.83	5	0.77	6	0.80	<0.01	NS	7	0.78	4	0.71	7	0.75	0.13	<0.01
	Dhps 7.7	6	0.79	7	0.84	8	0.82	0.09	<0.01	10	0.85	6	0.67	10	0.76	0.14	<0.01
	All loci	5	0.74	6	0.79	6	0.76	0.04	<0.01	9	0.81	5	0.67	9	0.74	0.11	<0.01
Neutral *loci*	TA1	5	0.79	8	0.87	8	0.83	0.04	NS	9	086	8	0.88	9	0.87	<0.01	NS
	TA102	5	0.78	9	0.87	9	0.83	0.04	0.01	6	0.75	7	0.86	7	0.80	<-0.01	NS
	ARA2	6	0.79	6	0.69	7	0.74	0.06	<0.01	7	0.80	6	0.82	7	0.81	-0.01	NS
	TA87	5	0.79	8	0.84	7	0.82	0.05	0.02	8	0.83	7	0.87	8	0.85	0.02	NS
	PfPK2	5	0.83	8	0.80	8	0.81	0.11	0.02	10	0.87	8	0.87	11	0.87	0.02	<0.05
	TA81	5	0.77	6	0.78	7	0.78	-0.02	NS	9	0.88	8	0.83	9	0.85	<0.01	NS
	TA42	1	0.00	4	0.29	3	0.14	0.03	NS	5	0.44	3	0.18	5	0.31	0.05	0.04
	Pfg377	3	0.56	4	0.59	3	0.57	<-0.01	NS	5	0.59	3	0.40	5	0.50	0.01	NS
	TA109	6	0.86	6	0.81	6	0.83	-0.02	NS	5	0.72	5	0.60	6	0.66	0.16	<0.01
	All loci	5	0.69	6	0.73	6	0.71	0.03	<0.01	7	0.75	6	0.70	7	0.72	0.03	0.01

N: number of isolates genotyped, He: expected heterozygosity; Rs: allelic richness. All loci: mean over loci Rs and He and global Fst over loci as calculated by FSTAT. P: P- values of permutation tests to assess significance of Fst values. NS: non-significant (P>0.05).

ISSN: 1475-2875