Development of a population suppression strain of the human malaria vector mosquito, Anopheles stephensi

Malaria Journal

Table 5 Effects of tetracycline on survival and flightless phenotype of transgenic Anopheles stephensi carrying driver and effector transgenes

♂ driver lines outcrossed with ♀ effector line E-1	Tetracycline concentration	Larvae		Transgenic adults
		Only one transgene	Effector + driver transgenes	Flying females	Flightless females	Flying males	Totals (% survival larvae-adult)
DAa-3	10 μg/ml	2535	371	108	16	226	350 (94%)
DAa-3	5 μg/ml	930	269	37	3	91	131 (49%)
DAa-3	1 μg/ml	610	143	21	10	69	100 (70%)
DAa-3	100 ng/ml	750	162	15	16	61	92 (57%)
DAa-3	10 ng/ml	600	127	9	28	38	75 (59%)
DAs-21	10 μg/ml	nd	233	25	18	82	125 (54%)
DAs-22	10 μg/ml	nd	628	155	12	285 (1)*	453 (72%)

*One flightless male was observed among the progeny of driver DAs-22 ♂ x effector E-1 ♀ cross (indicated in parenthesis).

ISSN: 1475-2875