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Table 2 Comparison of CSP-ELISA with qPCR and multiplex PCR for detection of Plasmodium falciparum sporozoites

From: Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae

 

CSP-ELISA positive

CSP-ELISA negative

Total

qPCR positive

261

15

276 (76%)

qPCR negative

41

47

88 (24%)

Total

302 (83%)

62 (17%)

364 (100%)

PCR positive

211

6

217 (60%)

PCR negative

91

56

147 (40%)

Total

302 (83%)

62 (17%)

364 (100%)

  1. McNemar test: CSP-ELISA vs qPCR, P <0.001; CSP-ELISA vs PCR, P <0.001.
  2. CSP-ELISA was performed on head-thorax-carcasses, and qPCR and multiplex PCR were performed on salivary glands DNA.