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Figure 1 | Malaria Journal

Figure 1

From: A novel, single-amplification PCR targeting mitochondrial genome highly sensitive and specific in diagnosing malaria among returned travellers in Bergen, Norway

Figure 1

Sensitivity of three different Plasmodium genus PCR protocols. A dilution series, run in 30 parallels, was prepared from a 200 p/μl stock of Plasmodium falciparum, US 04 F Nigeria XII. The 2% agarose gel picture shows a typical parallel for each assay; new single-round amplification mitochondrial PCR employing primers PgMt19 F3 & B3 [21](A), modified single-round amplification 18S PCR employing primers rPLU 6 & 5 [9](B), and gold standard genus-specific nested 18S PCR [10](C). The product sizes are 220 base pair (bp), 1200 bp, and 250 bp, respectively. Lane 1 = 100 bp DNA Ladder (New England BioLabs), lane 2 = 10 p/μl, 3 = 5 p/μl, 4 = 1 p/μl, 5 = 0.5 p/μl, 6 = 0.1 p/μl, 7 = 0.05 p/μl, 8 = 0.001 p/μl, and 9 = no template. The new mitochondrial PCR had more defined bands than the modified 18S PCR, and especially the nested 18S PCR.

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