Microsatellite genotyping of Plasmodium vivax infections and their relapses in pregnant and non-pregnant patients on the Thai-Myanmar border

Table 2 Genetic diversity of P. vivax infections in pregnant and non-pregnant women based on 8 microsatellite markers

Episodes	Diversity	Patients	n	Plasmodium vivax loci								Mean	SD	SE	p-value^a
				Pv 1.501	Pv 3.27	Pv 3.502	Pv 6.34	Pv 8.504	MS1	MS5	MS7
Admission samples	No. of distinct alleles (A)	P	18	9	9	7	7	6	5	6	6	6.88	1.46	0.52	0.265
	No. of distinct alleles (A)	NP	18	8	12	9	8	6	4	9	5	7.63	2.56	0.91
	H _e	P	18	0.919	0.895	0.889	0.739	0.856	0.772	0.838	0.742	0.831	0.07	0.03	0.533
	H _e	NP	18	0.876	0.948	0.922	0.830	0.739	0.767	0.892	0.802	0.847	0.07	0.03
	No. of distinct alleles per locus	P	18	1.59	1.61	1.50	1.22	1.56	1.35	1.27	1.38	1.44	0.14	0.05	<0.001
	No. of distinct alleles per locus	NP	18	1.28	1.33	1.11	1.17	1.22	1.13	1.13	1.07	1.18	0.09	0.03
Recurrent samples	No. of distinct alleles (A)	P	44	9	11	10	12	8	6	9	9	9.25	1.83	0.65	0.487
	No. of distinct alleles (A)	NP	36	13	14	10	11	8	6	8	8	9.63	2.76	0.98
	H _e	P	44	0.882	0.824	0.872	0.901	0.846	0.736	0.848	0.839	0.844	0.05	0.02	0.316
	H _e	NP	36	0.911	0.919	0.884	0.867	0.808	0.783	0.874	0.835	0.86	0.05	0.02
	No. of distinct alleles per locus	P	44	1.49	1.40	1.30	1.50	1.36	1.26	1.27	1.51	1.38	1.11	0.04	<0.001
	No. of distinct alleles per locus	NP	36	1.26	1.31	1.11	1.17	1.11	1.06	1.10	1.23	1.17	0.09	0.03
All samples	No of distinct alleles (A)	P	62	9	12	10	12	8	7	9	10	9.63	1.77	0.63	0.320
	No of distinct alleles (A)	NP	54	13	15	10	13	9	6	9	8	10.38	3.02	1.07
	H _e	P	62	0.885	0.846	0.869	0.864	0.845	0.736	0.834	0.820	0.837	0.05	0.02	0.340
	H _e	NP	54	0.906	0.921	0.888	0.848	0.781	0.770	0.866	0.836	0.852	0.06	0.02
	No. of distinct alleles per locus	P	62	1.52	1.46	1.35	1.42	1.42	1.28	1.29	1.47	1.40	0.09	0.03	<0.001
		NP	54	1.26	1.31	1.11	1.17	1.15	1.08	1.11	1.18	1.17	0.08	0.03

P = pregnant women and NP = non-pregnant women.
The No. of alleles and H_e values were calculated from a data set in which only the predominant alleles at each locus was considered.
The No. of alleles per locus values were calculated from all detected alleles at each loci, divided by the total number of samples.
^a Paired t-test was used to compare the means between groups.

ISSN: 1475-2875