Figure 1
From: Identification of piggyBac-mediated insertions in Plasmodium berghei by next generation sequencing
The piggyBac helper plasmid and donor plasmids for co-transfection of Plasmodium berghei parasites. The helper plasmid (left) contains the transposase gene under control of the constitutive eef1aa 5′UTR and the dhfr/ts 3′UTR for transient transposase expression. The two donor plasmids (right) contain the Pb dhfr/ts selectable marker and gfp fusion gene expression cassette under the control of constitutive dhfr/ts 5′UTR or eef1aa 5′UTR. The expression cassette is flanked by the piggyBac inverted terminal repeats (ITR).