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Figure 4 | Malaria Journal

Figure 4

From: Identification of piggyBac-mediated insertions in Plasmodium berghei by next generation sequencing

Figure 4

PCR amplification of all insertion sites identified by the ‘BLAST method’, the ‘SOAP method’ and the ‘iPCR method’. Half-nested PCR products were amplified by each of site-specific primers and a pair of primers located in either of the piggyBac ITR arms using the genomic DNA of post-transfection parasites as templates. The number of each lane coincides with the number shown in Additional file 1; L = DNA ladder.

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