Figure 4
From: Identification of piggyBac-mediated insertions in Plasmodium berghei by next generation sequencing
PCR amplification of all insertion sites identified by the ‘BLAST method’, the ‘SOAP method’ and the ‘iPCR method’. Half-nested PCR products were amplified by each of site-specific primers and a pair of primers located in either of the piggyBac ITR arms using the genomic DNA of post-transfection parasites as templates. The number of each lane coincides with the number shown in Additional file 1; L = DNA ladder.