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Table 3 Genetic diversity among PCR size classes and PCR-RFLP haplotypes for MSP-3α

From: Merozoite surface protein-3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

PCR-RFLP group Band size (bp) n Average pairwise divergence per site (SE) Geographic origin of isolates
At all informative sites among the group At sites shared by all isolates
Size Class 1 1884-1950 27 0.052 (0.003) 0.029 (0.003) Asia, Africa, South Americaa
Size Class 2 1590-1644 10 0.031 (0.002) 0.016 (0.002) Venezuela
Size Class 3 1449-1461 4 0.029 (0.002) 0.023 (0.003) Brazil, Thailand, Malaysia
Size Class 4 1137-1149 6 0.024 (0.002) 0.024 (0.003) Thailand, New Guinea
Size Class 5 1770 1 - - Indonesia
Haplotype 1.1 1896-1917 4 0.043 (0.003) 0.025 (0.003) Korea, Mauritania, Thailand, Brazil
Haplotype 1.2 1896-1920 3 0.048 (0.003) 0.030 (0.004) Thailand, Brazil
Haplotype 1.5 1929-1950 2 0.033 (0.003) 0.022 (0.004) Vietnam, India
Haplotype 1.8 1908-1926 2 0.058 (0.005) 0.031 (0.005) Panama, Indonesia
Haplotype 1.17 1884-1887 2 0.017 (0.002) 0.009 (0.002) Thailand, Brazil
Haplotype 2.1 1620 5 0.002 (0.001) 0.002 (0.001) Venezuela
Haplotype 3.2 2100 2 0.019 (0.003) 0.009 (0.003) Thailand
Haplotype 4.1 1137 5 0.016 (0.002) 0.017 (0.003) Thailand, New Guinea
  1. Nucleotide diversity for the 5 PCR band sizes and the 8 PCR-RFLP haplotypes with more than one sample (n) is shown. Due to the size differences between alleles, the number of informative sites differs between analyses; both diversity as calculated for all informative sites within the group and only at the sites shared by all isolates in the total sample population (n = 48, number of informative sites = 1413) are shown for comparison. a Only the continents for the 27 sequences in PCR amplification size class 1 are listed.