Table 2 Primer sequences and conditions for the PCR of the MIF gene
PCR set | Primer locations | Primer sequences (5′ - 3′) | Annealing temperature (°C) | PCR product size |
|---|---|---|---|---|
SET 1 | MIF-F (−1202) | GAGCAGTGGACACTCAGTCAGC | 65 | 600 bp |
MIF-R (−647) | CCTCTGGGCAACTTCAGCTCCT | |||
SET 2 | MIF-F (−740) | GCACCTGCTAGATGGTCCCCG | 65 | 696 bp |
MIF-R (−86) | AGTGGGGAAGTCACCGCCTG | |||
SET 3 | MIF-F (−315) | TTCATCTCTGGAAGGGTAAGGGG | 62 | 1370 bp |
MIF-R(+1009) | GACACTGGGGCTCCTCTGTTAGG | |||
MIF-Internal | AGTGGTGTCCGAGAAGTCAG | |||
SET 4 | MIF-F (+747) | TAAGAGCCGCAGGGACCCAC | 67 | 596Â bp |
MIF-R (+1302) | TGGCAGTGAGTGGCTCTGGG | |||
SET 5 | MIF-F (+1182) | GGGAGGAGGAGTTGGAGTTGGG | 65 | 600Â bp |
| Â | MIF-R (+1757) | CCCTGGAGCTTCTATTCTCCTTCCT | Â | Â |