Re-annotation of pvron4 within the PviS_CM000450 contig (above) schematic representation of PVX_091435 from the pvron4 gene in PlasmoDB and annotation suggested after bioinformatics and experimental analysis. The start positions for the ORFs within the contig are shown and the transcription direction. PVX_091435 was originally annotated as an 8,437 bp gene consisting of six exons. The re-annotation proposed has two ORFs where pvron4 start codon (shown in bold) is located 5,780 bp downstream the start codon initially reported in PlasmoDB. Amplification of the expected PCR products for pvron4 from gDNA and cDNA are shown on the agarose gel. Lane 1: amplification of ron4 from gDNA. Lane 2: amplification of ron4 from a sample of RNA from VCG-1 strain schizonts treated with reverse transcriptase (RT+). Lane 3: amplification of ron4 from a sample of RNA from VCG-1 schizonts without reverse transcriptase (RT-). MWM: molecular weight marker. Lane 4. Amplification of a pvron1 fragment from an RT+ sample. Lane 5: pvron1 amplification from an RT- sample.