Figure 2

Flow cytometric analysis of cells from peripheral blood on different days post-infection. BALB/c mice were infected ip with 10⁵P. yoelii 17X-GFP parasites and peripheral blood was analysed at different days post-infection. Cells were labelled with CD71 and TER119 specific antibodies and analysed for GFP transgenic parasites in a BD LSRFortessa Flow Cytometer. The gate was set to exclude debris and include erythroid cells. TER119⁺CD71⁺ cells were gated into two populations according to differences in CD71 intensity. (A) Percentages of CD71^hi, CD71^lo and CD71^- as a function of erythroid cells from blood are expressed as the mean ± SEM of three mice. (B) Percentages of infected cells (CD71^hiGFP⁺, CD71^loGFP⁺ and CD71^-GFP⁺) as a function of erythroid cells from blood are expressed as the mean ± SEM of three mice. (C) Parasitaemias and (D) percentages of infected cells (CD71^hiGFP⁺, CD71^loGFP⁺ and CD71^-GFP⁺) as a function of CD71^hi, CD71^lo and CD71^- respectively are expressed as the mean ± SEM of three mice. Data were evaluated by analysis of variance for each cell type in the different time points post-infection and versus non-infected (*P <0.05, **P <0.01 and ***P <0.001) (Dunnet post hoc test versus NI). Non-infected animals (NI); animals infected with the P. yoelii 17X nonlethal-GFP strain (NL).