Figure 3

Flow cytometric analysis of cells from erythopoietic organs on different days post-infection. BALB/c mice were infected ip with 10⁵P. yoelii 17X-GFP parasites and cells from bone marrow and spleen were analysed at different days post-infection. Cells were labelled with CD71 and TER119 specific antibodies and analysed for GFP transgenic parasites in a BD LSRFortessa Flow Cytometer. The gate was set to exclude debris and include non-nucleated erythroid cells. TER119⁺CD71⁺ cells were gated into two populations according to differences in CD71 intensity. Analyses of bone marrow (A, B) and spleen (C, D) erythroid cells. (A, C) Percentages of CD71^hi, CD71^lo and CD71^- as a function of erythroid cells are expressed as the mean ± SEM of three mice (B, D). Percentages of infected cells (CD71^hiGFP⁺, CD71^loGFP⁺ and CD71^-GFP⁺) as a function of erythroid cells are expressed as the mean ± SEM of three mice. Data were evaluated by analysis of variance for each cell type in the different time points post-infection and versus non-infected (*P <0.05, **P <0.01 and ***P <0.001) (Dunnet post hoc test versus NI). Non-infected animals (NI); animals infected with the P. yoelii 17X nonlethal-GFP strain (NL).