Figure 5
CD71hiimmature cells as a target for the development of the Plasmodium yoelii 17X-mCherry strain in vitro culture. (A) CD71 expression of blood cells from wild type mouse (black line) and a PKD mouse (grey line). Results were representative of three independent experiments. Percentages of CD71+ cells were 47,82% for PKD mice and 6,89% in control mice. CD71hi cells represent 24,40% for PKD mice and 4,92% for control mice. (B) Percentages of parasitaemia during in vitro culture of P. yoelii using PKD reticulocytes. Results were representative of four independent experiments. (C) Giemsa staining images of blood smears after 72, 96 and 120 hours of in vitro culture. (D) Representative dot plot of CFSE-PKD/mCherry-parasite signal for erythroid cells after 72 hours of in vitro culture. (E) Representative dot plot of CD71-PE/mCherry-parasite signal for erythroid cells after 72 hours of in vitro culture.