Table 1 Primer sequence for the amplification of pfdhfr and pfdhps
PCR reaction | Primer pair sequence | Expected size | Reference | |
|---|---|---|---|---|
pfdhfr | Nest 1 | Pfdhfr_D1 5′ TTTATATTTTCTCCTTTTTA 3′ | 718 bp | [9] |
Pfdhfr_D2 5′ CATTTTATTATTCGTTTTCT 3′ | ||||
Nest 2 | Pfdhfr_M1 5′ TTTATGATGGAACAAGTCTGC 3′ | 648 bp | ||
Pfdhfr_M5 5′ AGTATATACATCGCTAACAGA 3′ | ||||
pfdhps | Nest 1 | Pfdhps_N185 5′ TGATACCCGAATATAAGCATAATG 3′ | 1031 bp | [10] |
Pfdhps_N218 5′ ATAATAGCTGTAGGAAGCAATTG 3′ | ||||
Nest 2 | Pfdhps_Rc 5′ GGTATTTTTGTTGAACCTAAACG 3′ | 728 bp | ||
Pfdhfr_D2 5′ ATCCAATTGTGTGATTTGTCCAC 3′ | ||||