Figure 2

Schematic diagram representing full-length and truncated pf DHFR-TS. Constructs of pf DHFR domain containing amino acids 1–228 followed by truncated JR sequences of different lengths linked to the N-terminus of the pf TS domain were cloned into the pET15b expression vector. The numbers of amino acid are shown above the gene and the names of the mutants are indicated.