Figure 5
[3H]-FdUMP binding of crude extracts and partially purified proteins. Crude extracts and partially purified proteins from plasmids encoding pfDHFR co-transformed with pfJRTS mutants with truncated JR (panels A and B, respectively), and plasmids encoding pAC28 co-transformed with pfDHFR-TS with truncated JR (panel C and D, respectively) were labeled with [3H]-FdUMP. For panels A and B: lane 1, pf DHFR-TS (3D7); lane 2, pf JRTSâ–³232-235 co-expressed with pAC28-pf DHFR; lane 3, pf JRTSâ–³232-251 co-expressed with pAC28-pf DHFR; lane 4, pf JRTSâ–³232-265 co-expressed with pAC28-pf DHFR; lane 5, pf JRTSâ–³232-271 co-expressed with pAC28-pf DHFR; lane 6, pf JRTSâ–³232-274co-expressed with pAC28-pf DHFR; lane 7, pf JRTSâ–³232-276 co-expressed with pAC28-pf DHFR; lane 8, pf JRTSâ–³232-277 co-expressed with pAC28-pf DHFR; lane 9, pf JRTSâ–³232-299 co-expressed with pAC28-pf DHFR. For panels C and D: lane 1, pf DHFR-TS (3D7); lane 2, pf DHFR-TSâ–³229-265; lane 3, pf DHFR-TSâ–³229-271; lane 4, pf DHFR-TSâ–³229-274; lane 5, pf DHFR-TSâ–³229-275; lane 6, pf DHFR-TSâ–³229-276; lane 7, pf DHFR-TSâ–³229-277993. M is the molecular weight standard markers. (I) Coomassie blue stained SDS-PAGE, (II) autoradiogram of the SDS gel.