The percentage of splenic CD4+/CD8+T cells, and the absolutely numbers of CD4+CD25+Foxp3+Tregs, IL-10-secreting Tregs and IL-10+Foxp3-CD4+T cells in C57BL/6 mice co-infected with 100 or 200 S. japonicum cercariae and 1 × 106(A) or 1 × 105(B) P. berghei pRBCs eight weeks later. Intensities of S. japonicum cercariae in each panel are indicated as co-infection-200c and co-infection-100c, respectively. Mice infected with only P. berghei are indicated as P. berghei-mono. Mice were dissected on days 1, 3, 5, 8 post-P. berghei infection to obtain the respective cell counts. Bars represent the mean values ± SD. One-way ANOVA was performed to compare values between indicated groups with *indicating P < 0.05 and #indicating P < 0.01. n = 5–6 mice per group. FACS plots were used to show the cell populations and the gating strategy.