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Figure 4 | Malaria Journal

Figure 4

From: Identification of heat shock factor binding protein in Plasmodium falciparum

Figure 4

Pf HSBP undergoes nuclear translocation upon heat shock. (A) immunoblot analysis to examine Pf HSBP expression under normal and heat shock conditions reveals that Pf HSBP does not get heat induced; (B) immunoblot analysis of cytoplasmic and nuclear fractions to determine the localization of Pf HSBP under normal and heat shock conditions; (C) relative amount of Pf HSBP in cytoplasmic and nuclear compartments in normal and heat shock conditions. Upon heat shock amount of Pf HSBP in nucleus increases; (D) IFA to determine the localization of Pf HSBP under normal and heat shock conditions. (Cl - Control condition (37°C), HS - heat shock condition (42°C); C - cytoplasm, N - nucleus) reveals nuclear translocation of Pf HSBP upon heat shock.

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