Figure 4
From: Identification of heat shock factor binding protein in Plasmodium falciparum
Pf HSBP undergoes nuclear translocation upon heat shock. (A) immunoblot analysis to examine Pf HSBP expression under normal and heat shock conditions reveals that Pf HSBP does not get heat induced; (B) immunoblot analysis of cytoplasmic and nuclear fractions to determine the localization of Pf HSBP under normal and heat shock conditions; (C) relative amount of Pf HSBP in cytoplasmic and nuclear compartments in normal and heat shock conditions. Upon heat shock amount of Pf HSBP in nucleus increases; (D) IFA to determine the localization of Pf HSBP under normal and heat shock conditions. (Cl - Control condition (37°C), HS - heat shock condition (42°C); C - cytoplasm, N - nucleus) reveals nuclear translocation of Pf HSBP upon heat shock.