Figure 2

A typical electrophoregram showing Plasmodium species banding patterns after the Nest 2 reaction. In this experiment, a master mix was prepared to amplify Plasmodium DNA in all the samples in the Nest 1 reaction. After the Nest 2 amplification protocol, the amplicons were then separated on a 2% agarose gel (see methods for more details). M = 100 bp Ladder, F = P. falciparum, V = P. vivax, M = P. malariae, O = P. ovale. –ve ctrl = Negative control (MiliQ water), Pf + ve = P. falciparum positive control DNA, Pv + ve = P. vivax positive control DNA, BK095 & BK010 are 2 subjects’ samples.