Effect of ingested human antibodies induced by RTS, S/AS01 malaria vaccination in children on Plasmodium falciparum oocyst formation and sporogony in mosquitoes

Table 3 Transmission-blocking activities of human polyclonal IgGs fed six days post-infected blood meal ^a

Sample	Mean ooc^b	PIm[o]^c	p-value^d	Mosquito^e	PIp[o]^f	p-value^g	Mean Spz^h	PIm[s]ⁱ
CS-1	37.7	-13	0.82	0/20	0	1.00	91.1	-2
	(19, 82)	(-176, 54)			(-10, 16)
CS-2	33.7	-1	0.97	1/20	5	0.15	72.7	19
	(0, 54)	(-151, 63)			(-4, 24)
CS-3	35.8	-8	0.94	0/20	0	1.00	87.4	3
	(5, 72)	(-157, 59)			(-10, 16)
CS-4	34.6	-4	0.98	0/20	0	1.00	83.8	7
	(6, 74)	(-144, 61)			(-10, 16)
Contl-1	33.2	N/A	N/A	0/20	N/A	N/A	84.8	N/A
	(10, 62)
Contl-2	33.3	N/A	N/A	0/20	N/A	N/A	94.7	N/A
	(10, 77)

^aAll purified IgGs were tested at 3.75 mg/ml and fed six days post-infected blood meal.
^bArithmetic mean (range) of oocysts per mosquito midgut.
^cPer cent inhibition of mean oocyst intensity (PIm[o]) and the 95% confidence interval (95% CI).
^dTwo-sided p-values for testing whether PIm[o] is significantly different from 0.
^eNumber of mosquitoes without any oocysts/number of mosquitoes examined.
^fPer cent inhibition of prevalence of mosquitoes with oocysts (PIp[o]) and the 95% CI.
^gp-values of PIp[o].
^hNumber of sporozoites (×10³) per mosquito calculated from a pool of salivary glands of 12 mosquitoes.
ⁱPer cent inhibition of mean sporozoite intensity (PIm[s]) calculated with the average of sporozoites counts of Contl-1 and Contl-2.

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