Figure 1

Pb SUB2 homology models identify peptide targets for immunization. (A) Cartoon (left) or surface representation (right) homology model of the Pb SUB2 catalytic domain (residues L672-L971) visualized with PyMOL software. Regions corresponding to Peptide #1 (purple) and Peptide #2 (green) were used for immunization experiments. Catalytic residues Asp705, His748 and S911 in the active site pocket are shown as orange, cyan and red spheres, respectively. (B) Lateral view of Peptide #1 (purple) and Peptide #2 (green) in the Pb SUB2 surface representation model reveals that each peptide corresponds to solvent exposed areas (left). Sequence alignments of both peptide sequences with corresponding regions of P. falciparum, P. vivax, P. knowlesi, and P. yoelii SUB2 (right). The amino acid position of the first and last residues of each peptide sequence with respect to full length Pb SUB2 are shown at the top left and right corner of each alignment. Conserved residues are highlighted with a red background and regions of similarity are marked with red letters against white background. Sequence alignments were visualized using ESPript [21].