Figure 2

Production of recombinant SUB2 and recognition using Sub2 immune sera. (A) Domains of endogenous Pb SUB2 (top): signal peptide (residues 1–20), pro-domain (residues 21–626), catalytic domain (residues 627–1,088) with catalytic residues Asp (orange), His (cyan) and Ser (red), transmembrane domain (residues 1,089–1,111) and cytoplasmic tail (residues 1,112–1,230). Representation of recombinant Pb SUB2 (middle) containing a minimal inhibitory domain and the full catalytic domain. Below, Pb SUB2 Peptides #1 (purple) and #2 (green) are aligned to endogenous Pb SUB2 and rPb SUB2 with peptide sequences. Illustrations were prepared with DOG1.0 [22]. (B) Recombinant proteins maltose binding protein (MBP), Pb SUB2 or Pf SUB2 MBP-fusion proteins were separated on polyacrylamide gels and stained with Coomassie, or transferred and visualized by Western Blot with specific MBP, SUB2, or KLH antibodies. Arrows denote full length Pb SUB2 and Pf SUB2 recombinant products. Approximate sizes in kilodaltons (kDa) are displayed on the left.