PbHT localizes to the male gamete surface. The P. berghei HT-myc line was labelled with an α-tubulin antibody (red), an anti-c-myc antibody (green) and stained with DAPI (blue). (A) 3-D projection of a male gametocyte in the process of exflagellation. Scale bar 5 μm. (B) Enlarged inset from (A) displaying the distribution PbHT-myc and α-tubulin in sequential Z stacks (0.1 μm). Scale bar 1 μm. PbHT-myc is abundant and localized to the periphery of male gametes.