Figure 6

Coenzyme A supplementation reduces the antiplasmodial effect in a dose-respondent manner. A: Eight point dose-response curves of hit compounds with (green) and without (black) supplementation of CoA. The optimal CoA concentration for the chemical rescue was determined for each CoA batch and ranged between 0.8-2 mM, once determined the CoA concentration within each experiment was fixed. Generally a shift towards reduced antiplasmodial activity was observed, when CoA was supplemented. Curves represent n = 2 in duplicate point each; error bars indicate +/- standard error of mean (SEM). B: Eight point dose-response curves of control compounds with (green) and without (black) supplementation of CoA. The optimal CoA concentration was determined for each CoA batch; once determined the CoA concentration within each experiment was fixed. The activity of the CoA pathway unrelated known anti-malarials artemisinin and pyrimethamine was not reduced by CoA supplementation, whereas the activity of the known CoA pathway inhibitor panthenol was counteracted by CoA addition. Curves represent n = 2 in duplicate point each; error bars indicate +/- standard error of mean (SEM).