Molecular surveillance of pvdhfr, pvdhps, and pvmdr-1 mutations in Plasmodium vivax isolates from Yunnan and Anhui provinces of China

Table 1 Sequences of primers and cycling conditions used to amplify pvdhfr , pvdhps , and pvmdr-1 of Plasmodium vivax isolates ^a,b

Genes^c	Primers^d	PCR cycling conditions^e	Product size (bp)	Reference
pvdhfr (P)	F: 5’- CACCGCACCAGTTGATTCCT-3’	95°C 5 min/[95°C 30 s, 58°C 30 s, 68°C 1 min] × 30 cycles, 68°C 7 min	979	[16]
pvdhfr (P)	R: 5’- CCTCGGCGTTGTTCTTCT-3’		979	[16]
pvdhfr (S)	F: 5’- CCCCACCAC ATA ACG AAG-3’	95°C 5 min/[95°C 30 s, 58°C 30 s, 68°C 45 s] × 30 cycles, 68°C 7 min	755	[16]
pvdhfr (S)	R: 5’- CCC CACCTT GCTGTA AACC-3’		755	[16]
pvdhps (P)	F: 5’-GATGGCGGTTTATTTGTCG-3’	95°C 5 min/[95°C 30 s, 59°C 30 s, 68°C 1 min] × 30 cycles, 68°C 7 min	1009	[16]
pvdhps (P)	R: 5’-GCTGATCTTTGTCTTGACG-3’		1009	[16]
pvdhps (S)	F: 5’-GCTGTGGAGAGGATGTTC-3’	95°C 5 min/[94°C 30 s, 59°C 30 s, 68°C 45 s] × 30 cycles, 68°C 7 min	731	[16]
pvdhps (S)	R: 5’-CCGCTCATCAGTCTGCAC-3’		731	[16]
pvmdr-1 (P)	F: 5’-ACGACATGATCCAAACGACA-3’	94°C 2 min/[94°C 30 s, 60°C 30 s, 72°C 3 min] × 35 cycles, 72°C 5 min	2784	[28]
pvmdr-1 (P)	R: 5’-CTTATATACGCCGTCCTGCAC-3’		2784	[28]
pvmdr-1 (S)	F: 5’-GGATAGTCATGCCCCAGGATTG-3’	94°C 10 min/[94°C 40 s, 62°C 1 min, 72°C 1 min] × 35 cycles, 72°C 10 min	604	[15]
pvmdr-1 (S)	R: 5’-CATCAACTTCCCGGCGTAGC-3’		604	[15]

^aP. vivax isolates from Yunnan and Anhui provinces.
^bThe data provided oligonucleotide primers and cycling conditions of pvdhfr, pvdhps, and pvmdr-1 genes.
^cP, Primary PCR reaction; S, Secondary PCR reaction.
^dF, Forward primer; R, Reverse primer.
^eCycling conditions were modified in the present study.

ISSN: 1475-2875