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Figure 2 | Malaria Journal

Figure 2

From: Humanized HLA-DR4.RagKO.IL2RγcKO.NOD (DRAG) mice sustain the complex vertebrate life cycle of Plasmodium falciparum malaria

Figure 2

DRAG mice develop human Kupffer cells and liver endothelial cells. A) Immunostaining of livers using anti-human CD68 (green fluorescence) and counterstained with DAPI (DNA, blue fluorescence). B) Frequency of human Kupffer cells in livers of DRAG mice as determined by FACS using anti-human CD45 and anti-human CD68 on cells gated on the FSC/SSC monocyte region. Middle and right panels show CD68 staining on human (gated CD45+) cells and mouse (gated CD45) cells isolated from livers of HSC-infused DRAG mice. Data represent mean and interval frequencies from 4 mice analysed individually. Left panel shows binding of anti-human CD68 to monocytes from human peripheral blood. C) Immunostaining of livers using anti-human CD36 and counterstained with DAPI (DNA, blue fluorescence) showing presence of human endothelial cells in veins and sinusoids.

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