DRAG mice develop human Kupffer cells and liver endothelial cells. A) Immunostaining of livers using anti-human CD68 (green fluorescence) and counterstained with DAPI (DNA, blue fluorescence). B) Frequency of human Kupffer cells in livers of DRAG mice as determined by FACS using anti-human CD45 and anti-human CD68 on cells gated on the FSC/SSC monocyte region. Middle and right panels show CD68 staining on human (gated CD45+) cells and mouse (gated CD45−) cells isolated from livers of HSC-infused DRAG mice. Data represent mean and interval frequencies from 4 mice analysed individually. Left panel shows binding of anti-human CD68 to monocytes from human peripheral blood. C) Immunostaining of livers using anti-human CD36 and counterstained with DAPI (DNA, blue fluorescence) showing presence of human endothelial cells in veins and sinusoids.