Figure 5

Linearity of parasitaemia and monocyte proportions estimations by the tri-colour technique. Plasmodium falciparum NF54 asynchronous culture of ~30% parasitaemia and 2.5% haematocrit was mixed with ~26% human monocyte and titrated two-fold with 2.5% haematocrit. Plots of: A) measured parasitaemia by the tri-colour technique versus expected parasitaemia by microscopy and B) measured monocytes proportions by the tri-colour technique versus expected theoretical monocyte proportions from serial dilutions are shown, respectively. There was a strong linearity in the estimation of both monocyte proportions (r = 1.00, p < 0.0001) and parasitaemia (r = 0.99995, p < 0.0001). All values are plotted ± standard deviation for triplicate wells.