Figure 2

Parasitaemia courses after intranasal administration of artesunate. On day 5 pi, 6 pi or 7 pi, mice received a single IN administration of artesunate (20 mg/kg). A control group received IN placebo solution either on day 5 pi, 6 pi or 7 pi. In all treated groups, the treatment administration led to a significant decrease of parasitaemia by 88.7% (61.8-100%) within 24 hours. Parasitaemia were compared using ANOVA on day 5 pi and Kruskal-Wallis test on day 6 pi, 7 pi and 8 pi. In control group, mice were mostly dead on day 8 pi (10/13) and none of them was alive on day 11 pi. ***p <0.001, **p <0.01, NS: non-significant coloured arrows indicates the time of treatment for each group, black line: control group, blue line: mice treated at day 5, red line: mice treated at day 6, green line: mice treated at day 7.