Conventions for terms of the cassette. This figure considers the most common malaria RDT i.e. a three-band RDT targeting two antigens (P. falciparum and pan-Plasmodium antigen) in a two-step procedure (add specimen, next add buffer) with a cassette showing separated specimen and buffer wells. The following convention of terms is used: proximal (closest to the specimen and buffer wells) and distal (at the end of the migration [absorption] pad). Considering the cassette in a vertical view (with direction of the specimen/buffer flow “upwards”), there is the right hand side and the left hand side.