Figure 1

Schematic structure, full-length alignment sequence, and overlapping 20 mers covering D and C regions of MSP2. A) Represents schematic structures of merozoite surface protein 2 (MSP2) with different domains (modified from Flueck et al.[4]). Mature MSP2 consists about 200–250 amino acids and contains two conserved domains (black, N-and C-termini) flanking a polymorphic region that includes repetitive sequences (light grey) and a non-repetitive dimorphic domain (dark grey). The lengths of the non-conserved domains are strain-dependent. The dimorphic region defines two allelic families of MSP2, 3D7 and FC27. B) Shows sequence of the two allelic full-length sequences (from N-to C-terminal) of MSP2 (3D7 and FC27). Non-repeat family-dimorphic regions are shaded in light gray, conserved (N- and C-termini) sequences are underlined. The non-shaded and non-underlined sequences represent the polymorphic regions. C) Shows sequence of Dimorphic and constant C-region (blue) sequences, and overlapping 20 mers covering the two regions. In parentheses is the sequence position number. LSP: long synthetic peptide.