Low genetic diversity and functional constraint in loci encoding Plasmodium vivax P12 and P38 proteins in the Colombian population

Table 5 McDonald-Kreitman test for evaluating the action of natural selection

		P. vivax/P. cynomolgi			P. vivax/P. knowlesi
Worldwide isolates
		Fixed	Polymorphic	P_N/P_S> D_N/D_Sp-values	Fixed	Polymorphic	P_N/P_S> D_N/D_Sp-values
pv12	Non-synonymous substitutions	78.66	4	0.002	93.86	4	0.000
pv12	Synonymous substitutions	190.23	0	0.002	340.47	0
pv38	Non-synonymous substitutions	85.90	6	0.004	85.14	6	0.003
	Synonymous substitutions	257.31	3		265.94	3
Colombian population
pv12	Non-synonymous substitutions	93.05	1	0.146	115.54	1	0.083
pv12	Synonymous substitutions	197.20	0	0.146	347.80	0
pv38	Non-synonymous substitutions	89.22	5	0.023	88.50	5	0.016
	Synonymous substitutions	248.66	3		264.90	3

The McDonald-Kreitman test was done using sequences obtained from databases (worldwide isolates) together with Colombian ones, and just with those obtained in the Colombian population. The interspecies divergence data were obtained from comparing Plasmodium vivax sequences with two related species: Plasmodium cynomolgi and Plasmodium knowlesi. Significant values are shown in italics.

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