Reaction sensitivity of the recombinase polymerase amplification. A serial dilution of genomic DNA from P. falciparum 3D7 was used to test the analytical sensitivity of the assay. Positive RPA reaction products (174 bp) can be detect on a stained agarose gel (2%) (A). In the lateral flow format (LF-RPA) the sensitivity was 100 fg of genomic DNA (B). NTC: no template controls contained water.