Volume 13 Supplement 1

Challenges in malaria research: Core science and innovation

Open Access

Distinct HLA class II alleles influence antibody response to the Plasmodium vivax Duffy binding protein

  • Flora S Kano1,
  • Flávia A Souza-Silva1,
  • Taís N Sousa1,
  • Jéssica RS Alves1,
  • Michaelis L Tang1,
  • Roberto S Rocha1, 2,
  • Cristiana FA Brito1,
  • Ana Maria Sell3 and
  • Luzia H Carvalho1
Malaria Journal201413(Suppl 1):P51


Published: 22 September 2014

Plasmodium vivax infects human reticulocytes through a major pathway that requires interaction between an apical parasite protein, the Duffy binding protein (region II, DBPII) and its cognate receptor on reticulocytes, the Duffy antigen receptor for chemokines (DARC). Although most people naturally exposed to P. vivax fail to develop antibodies that inhibit the DBPII-DARC interaction, the genetic factors that modulate humoral responsiveness are poorly characterized. Aiming to investigate if DBPII non-responsiveness could be HLA class II-linked (DRB1, DQB1 and DQA1), we carried-out an open cohort study among 340 non-related volunteers in an agricultural settlement of the Brazilian Amazon; three cross-sectional surveys were conducted at 6-month intervals, and 240 out of 340 (71%) subjects had consecutive samples. At enrolment, 40.9% of the study population had DBPII IgG antibodies, as measured by conventional serology (ELISA); two alleles at HLA-DR locus (DRB1*10:01 and DRB1*14:02) were negatively associated with the antibody response, while two alleles at HLA-DQ locus (DQB1*02:02 and DQA1*01:03) were positively associated with antibody response. The 12-month follow-up study confirmed that those alleles associated with positive antibody response were also associated with the persistence of this specific antibody response. Further, we investigated whether HLA class II polymorphisms would influence the functional proprieties of DBPII antibodies (BIAbs, binding inhibitory antibodies), as assessed by the COS-7 cytoadherence assay. The results demonstrated that four alleles were associated with the presence (DRB1*07:01; DQB1*02:02, DQA1*02:01) or absence (DRB1*16:02) of DBPII BIAbs response. Taken together, this is the first demonstration that HLA class II polymorphisms might influence the frequency and stability of the DBPII immune response. Due to the relevance of these findings for vaccines now in development, it would be pertinent to investigate whether such an association exists in other P. vivax malaria endemic areas.



Supported by: the Research Foundation of Minas Gerais State (FAPEMIG), The Brazilian National Research Council (CNPq), Oswaldo Cruz Foundation (FIOCRUZ/PAPES VI), Laboratório de Imunogenética (UEM/PR).

Authors’ Affiliations

Centro de Pesquisas René Rachou/FIOCRUZ
Centro de Pesquisas Leonidas e Maria Deane/FIOCRUZ
Universidade Estadual de Maringá


© Kano et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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