Volume 13 Supplement 1

Challenges in malaria research: Core science and innovation

Open Access

Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum

  • Somdet Srichairatanakool1,
  • Supalerk Thipubol1,
  • Wachiraporn Tipsuwan2 and
  • Chairat Uthaipibull3
Malaria Journal201413(Suppl 1):P84

https://doi.org/10.1186/1475-2875-13-S1-P84

Published: 22 September 2014

Background

Iron is an essential micronutrient required by all living organisms including malaria parasites (Plasmodium spp.) for many biochemical reactions, especially growth and multiplication processes. Therefore, the malaria parasites needs to take up the iron from outside or/and inside the parasitized red blood cells (PRBC). Iron chelators are widely used for treatment of thalassemia-related iron overload and also inhibit parasite growth at levels which are non-toxic to mammalian cells.

Materials and methods

The inhibitory effect of 1-(N-acetyl-6-amino-hexyl)-3-hydroxypyridin-4-one (CM1) and green tea extract (GTE) on the growth of malaria parasite P. falciparum was investigated compared with standard chelators including desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX). A flow cytometric technique was used to enumerate the PRBC stained with SYBR Green I fluorescent dye. Labile iron pool (LIP) was assayed using calcein-acetomethoxy (Calcein-AM) fluorescent method.

Results

The IC50 values of DFO, GTE, CM1, DFX and DFP against P. falciparum were 14.09, 21.11, 35.14, 44.71 and 58.25 μM, respectively. Importantly, CM1 was more effective in reducing LIP level in the P. falciparum culture than DFP (P < 0.05).

Conclusions

CM1 and GTE exhibit anti-malarial activity. They could interfere with uptake of exogenous iron or deplete intracellular labile iron in malaria parasites, leading to inhibition of their growth.

Declarations

Acknowledgements

The work has been supported by the Thailand Graduate Institute of Science and Technology (TGIST), National Science and Technology Development Agency (NSTDA) and Faculty of Medicine, Chiang Mai University.

Authors’ Affiliations

(1)
Chiang Mai University
(2)
University of Phayao
(3)
National Science and Technology Development Agency

Copyright

© Srichairatanakool et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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