Table 2 Method for detecting codon 268 mutations: primer pairs for amplification with size of amplification product and restriction enzyme for following digest
Codon 268 | Type | Primer pair | Product size | Cutting enzyme | Fragment length |
|---|---|---|---|---|---|
Outer PCR | CYTb1 + CYTb2 | 939 bp | |||
TAT | Wild type | CYTb3 + CYTb5 | 384 bp | NsiI | 359 + 25 |
TCT | Y268S | CYTb2 + CYTb6 | 171 bp | AlwNI | 147 + 24 |
AAT | Y268N | CYTb2 + CYTb7 | 174 bp | SspI | 150 + 24 |