Skip to main content

Table 2 VH and VL assignments and alignment of CDR 3 sequences The selected (NP 04, 12, 13 designated Pf NPNA-1 bind to the repeat epitope), all other NP clones were randomly picked after the panning procedure and were subsequently shown not to be reactive with the repeat epitope. Non-selected (R01-10) were randomly picked from the library prior to initiating panning. The peptide sequence of the heavy and light chain complementarity-determining region 3 (CDR3) is shown below. VH/VL families, segments and the number of differences from germline segments were determined by using the V BASE sequence directory (Tomlinson, I. M., Williams, S. C., Corbett, S. J., Cox, J. P. L. & Winter, G., MRC Centre for Protein Engineering, Cambridge, UK) and the DNAPLOT alignment package (Müller, W. & Althaus, H.-H., Köln University)

From: Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor

clone code* VH family VH Segment Differences from germline VHCDR3 VL family VL Segment Differences from germline VLCDR3
PfNPNAl VH3 DP46 10 DRDSSSYFDS VkI L12a 15 QQYNSYSGLT
NP04, NP12, NP13 VH3 DP46 10 DRDSSSYFDS VkI L12a   QQYNSYSGLT
R01 VH1 4M28†‡ 28(+6)* §- DSESVAQWRY VkIV DPK24 43 QQSLSPVWT
R02 VH3 COS-3 27 (+3)_ GVNWCSDY VkI DPK9 10 QQSYSTSWT
R03 VH5 DP73 35 LYTSIYYFDS VkIV DPK24 7 QQYYSTPLT
R04 VH3 DP46 8 DRVTNFWSGYFDY VkIII DPK22 13 QQYGSSPGFT
R05 VH3 DP58 23 DSTVKTVTKMRYGLD V VkIII DPK22 8 QQYGSSPFT
R06 VH1 4M28 12 DNYGDPGGGFDI VkIII DPK22 11 QQYGNSPRT
R07 VH5 DP73 9 RFWFGELYDAFDI VkIV DPK24 16 HQYYSTPQT
R08 VH5 DP73 34 LYTSIYYFDS VkIII DPK22 14 QQYGRSPWT
R09 VH3 V3-21 34 DQGGGWSSEVDS VkIII Vg 5 QQRSNWPLT
R10 VH1 DP7 21 (+9)** ALYGHDAFDI VkI DPK4 12 PKYNSALHT
NP02 VH3 DP47 36 ERPYDAFDS VkIII DPK22 23 QQYSTSPPMYN
NP03 VH5 DP73 40 LYTSIYYFDS VkIII Vg 17 KQRSKWPPIT
NP05 VH3 V3-48 14 EPRGAGTTLYFDY VkIII DPK22 22 QQYGGSPGYN
NP08 VH4 4.30 18 DRGVSSGWTFDC VkII DPK16 32 MQLTAFPWT
NP09 VH4 DP71 17 FRGGVAAGYDY VkIII DPK22 24 QHYRESCS
NP10 VH4 DP78 29 DRVRVPYYYIDV VkIII DPK22 15 QQYGTSPYS
NP11 VH3 VH3-8 12 DTTVTHYFDY VkI DPK9 21 QQSFSSPRT
NP14 VH1 DP88 20 GPGATIHYYYMDV VkI DPK8 18 QQLDNYPLT
NP15 VH5 DP73 36 LYTSIYYFDS VkIII DPK22 28 QQYGNSPPT
  1. *Phage clones were either selected from the library at random (prefix R) or after four rounds of panning against (NPNA)3C-BSA, eluting with free (NPNA)3 peptide (prefix NP). The segment given the best DNAPLOT match, although the segment sequence has not been verified by duplication. Aligned after removal of the unusual sequence additions (see§,-, ‡,-, **). § Figure in brackets indicates an unusual sequence addition. - Sequence has two additional codons in CDR1. - Sequence has one additional codon in CDR1. ** Sequence has three additional codons in CDR2.