IgG recognition profiles of parasite clone FCR3 before and after selection for adhesion to CSA. Following selection of parasite clone FCR3 for adhesion to CSA the expression of variant surface antigens was investigated using FACS. 5000–10000 late stage parasites were gated using ethidium bromide and FITC fluorescence due to serum IgG binding was measured. Serum samples from six Scottish malaria naïve individuals were pooled and used as a control (IgGcontrol). Sera from 20 Ghanaian men were pooled to produce the malaria exposed male serum pool (IgGmale). Sera collected at the time of birth from the placentas of 15 Ghanaian women were pooled to produce the malaria exposed pregnant female serum pool (IgGpreg). The bar chart shows mean and standard error of the means for five independent experiments.