Table 1 Sequences of the digoxigenin-conjugated sequence-specific oligonucleotide probes (SSOPs). The SSOP-ID named the sequences used in detection of the haemoglobin and G6PD single nucleotide polymorphisms (SNPs).
SSOP ID | SSOP sequence1 | Nucleotide substitution | Amino acid substitution |
|---|---|---|---|
HbB | Â | Â | Â |
HbA-6: | 5' TGACTCCTGAGGAGAAGT | Â | Â |
HbS-6: | 5' TGACTCCTGT GGAGAAGT | A18T | E6V |
HbC-6: | 5' TGACTCCTA AGGAGAAGT | G17A | E6K |
HbA-26 | 5' TTGGTGGTGAGGCCCTGG | Â | Â |
HbE-26: | 5' TTGGTGGTA AGGCCCTGG | G77A | E26K |
G6PD | Â | Â | Â |
68V | 5' CACCTTCATCGTGGGCTA | Â | Â |
68M | 5' CACCTTCATCA TGGGCTA | G202A | V68M |
126N | 5' GCCACATGAATGCCCTCC | Â | Â |
126D | 5' GCCACATGG ATGCCCTCC | A376G | N126D |