Table 2 Primer sequences. Lower case nucleotide indicates the intentional mismatch, nucleotides in bold are located at site of SNP (where applicable), F and R indicate forward and reverse orientation.
An. gambiae species ID | (5' to 3')Fragment (size bp) | ||
|---|---|---|---|
IMP-UN: | F | GCTGCGAGTTGTAGAGATGCG | Â |
| Â | R | GCATGTCCACCAACGTAAAtCC | An. quadriannulatus (637) |
QD-3T | R | CAACCCACTCCCTTGACGaTG | An. melas and merus (529) |
ME-3T | R | GCTTACTGGTTTGGTCGGCAtGT | An. gambiae (464) |
GA-3T AR-3T | R | GTGTTAAGTGTCCTTCTCCgTC | An. arabiensis (388) |
Mopti/Savanna rDNA typing | |||
M5 | F | CTTGGTCTGGAGACCGTTCCaTA | Mopti (426) |
M3 | R | GACACGTCAACTAAGTCAACACATtAC | Â |
S5 | F | GCCCCTTCCTCGATGGaGC | Savanna (335) |
S3 | R | CAACCGGCCCAAACGGcTT | Â |
Rdl mutation assay | |||
RDLF | F | AGTTTGTACGTTCGATGGGTTA | positive control (256) |
RDLR | R | CCAGCAGACTGGCAAATACC | Â |
AARDL | F | GCTACACCAGCACGTGaTT | dieldrin resistant (158) |
RDLSS | R | CAAGACAGTAGTTACACCTAAaGC | dieldrin susceptible (121) |